Journal: bioRxiv
Article Title: The ALS- and FTD-associated proteins Annexin A11 and CHMP2B act sequentially in membrane repair
doi: 10.1101/2024.11.19.624330
Figure Lengend Snippet: A . Representative image sequence of CAL-51 cells stably expressing CHMP2B-L-GFP and transiently transfected with MPAct-mRuby3 to illuminate membrane proximal F-actin and subject to a single multi-photon laser ablation at the plasma membrane. Scale bars represent 10 μm. White arrow indicates area of ablation. Images representative of n = 5 cases where clear CHMP2B-L-GFP recruitment was observed, but with no MPAct-mRuby3 relocalisation. See corresponding movie S25. B . Representative image sequence from CAL-51 cells stably expressing CHMP2B-L-GFP, pretreated with cytochalasin D (2 μg/mL, 30 minutes prior) and subject to a single multi-photon laser ablation at the plasma membrane. Scale bars represent 10 μm. Arrowhead indicates area of ablation. Images representative of n = 7 cases where clear CHMP2B-L-GFP recruitment was observed. See corresponding movie S26. C . Representative image sequence from CAL-51 cells stably expressing CHMP2B-L-GFP, transiently transfected with a plasmid encoding LAMP1-tdTomato and subject to a single multi-photon laser ablation at the plasma membrane. Images representative of n = 7 movies where CHMP2B-L-GFP recruitment, but not LAMP1-mCh recruitment, was observed. Scale bars represent 5 μm. White arrow indicates area of ablation. See corresponding movie S27.
Article Snippet: A plasmid encoding MP-Act-mRuby3 was a kind gift from Prof Tobias Meyer (Stanford) via Addgene (155221).
Techniques: Sequencing, Stable Transfection, Expressing, Transfection, Membrane, Plasmid Preparation
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